CDS
| Accession Number | TCMCG077C03166 |
| gbkey | CDS |
| Protein Id | KAF5728422.1 |
| Location | complement(join(4849603..4849995,4850205..4850321,4851200..4851318,4851536..4851644,4851754..4851792,4852084..4852162,4852290..4852364,4852552..4852616,4853094..4853149,4853333..4853425,4853759..4854023)) |
| Organism | Tripterygium wilfordii |
| locus_tag | HS088_TW21G00569 |
Protein
| Length | 469aa |
| Molecule type | protein |
| Topology | linear |
| Data_file_division | PLN |
| dblink | BioProject:PRJNA542587, BioSample:SAMN11634134 |
| db_source | JAAARO010000021.1 |
| Definition | putative ubiquitin-activating enzyme E1 [Tripterygium wilfordii] |
| Locus_tag | HS088_TW21G00569 |
EGGNOG-MAPPER Annotation
| COG_category | H |
| Description | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor |
| KEGG_TC | - |
| KEGG_Module | - |
| KEGG_Reaction |
R07459
[VIEW IN KEGG] R07461 [VIEW IN KEGG] |
| KEGG_rclass |
RC00043
[VIEW IN KEGG] |
| BRITE |
ko00000
[VIEW IN KEGG] ko00001 [VIEW IN KEGG] ko01000 [VIEW IN KEGG] ko03016 [VIEW IN KEGG] ko04121 [VIEW IN KEGG] |
| KEGG_ko |
ko:K11996
[VIEW IN KEGG] |
| EC |
2.7.7.80
[VIEW IN KEGG]
[VIEW IN INGREDIENT] 2.8.1.11 [VIEW IN KEGG] [VIEW IN INGREDIENT] |
| KEGG_Pathway |
ko04122
[VIEW IN KEGG] map04122 [VIEW IN KEGG] |
| GOs |
GO:0003674
[VIEW IN EMBL-EBI] GO:0003824 [VIEW IN EMBL-EBI] GO:0005575 [VIEW IN EMBL-EBI] GO:0005622 [VIEW IN EMBL-EBI] GO:0005623 [VIEW IN EMBL-EBI] GO:0005737 [VIEW IN EMBL-EBI] GO:0008265 [VIEW IN EMBL-EBI] GO:0016740 [VIEW IN EMBL-EBI] GO:0016782 [VIEW IN EMBL-EBI] GO:0016783 [VIEW IN EMBL-EBI] GO:0044424 [VIEW IN EMBL-EBI] GO:0044464 [VIEW IN EMBL-EBI] |
Sequence
| CDS: ATGGATTCAAACGGAGGAGGAGAAGTGTGTAGGATCCTCCGCGAACTCAAGACCTTAAAGGCATCTAAGAGCGACATCGAGCGTCGAATTTCGGACCTAGAAGCTCAGCTCCGGGAGCTAAATCTCAACAACGGCACCGTCGTTCCTAATGGTTCCTCCTGCACTCTAATGTCCACCGTTGATTCTGGTTTTGGTCATGGACTTTCGGCGGATATGATTTACCGATATAGTCGCCAGCTCTTGATTCCTTCATTCGGTGTCCAAGCTCAATCAAATCTATTAAAGTCTTCAATTTTAGTTGTTGGAGCTGGGGGGTTGGGCTCGCCTGCCCTGCTATATCTTGCAGCTTGTGGTGTTGGTCGCTTAGGTATTGTTGATCATGATGTGGTTGAGCTAAATAATATGCACAGACAGATTATACACACGGAAGCATATATTGGCCAACCAAAAGTGAAGTCTGCTGCTGCTTCCTGTCGCTCGATCAATTCCACGATACAAATTGTGGAACACAAAGAAGCTTTATGCGCATCCAATGCCTTGGAAATTTTGAGCAAGTATGACATAATAGTAGATGCAACAGATAATGCACCAAGTCGTTACATGATCAGTGATTGCTGCGTAGTGTTGGGAAAGCCTCTTGTATCTGGTGCTGCAGTGGGATTGGAAGGACAGCTTACAGTCTATAACTACAATGGAGGTCCGTGCTATCGGTGCCTATTTCCAACTCCACCACCTACAACAGCGTGTCAAAGATGTTCTGACAGTGGAGTTCTAGGAGTGGTTCCTGGTGTCATTGGTGTCCTTCAAGCACTAGAGACCATTAAAATTGCTGCAGCTGTTGGTGAACCACTCTCGGGCCGGATGCTTTTGTTTGATGCATTGTCAGCAAGGATTCGTATTGTCAAAATTAGAGGCAGGTCATTGCAATGTGAAGTTTGTGGAGAAAACACGGCATTCACCCAGAAGCAATTCAGTGAATTTGATTATGAGAATTTCACTCAATCCCCATTATCAACATCTCCATTGAAGTTAAACCTGCTTCCGGCAGATAGTAGAATAAGCAGCAAGGAGTTCAAAGAGAGAATCGTTAATGGTGAAGCAAATGTGTTGGTTGATGTACGCCCCCCGCACCATTACAAGATTGTTTCCCTTCCCGGGTCCGTGAACATCCCATTGTCAAGATTGGAGTCCAGGTTGCCAGAAATCTCTTCATTTTTAAATGAAACAAAAGAGCGCAAGGCGTTGAATCCTGATTCAGTTCCTAGCCTCTATGTAATATGTAGAAGAGGTAATGATTCACAGAGAGCTGTTCAGCTCCTCCACAAGATGGGTTTCACCATAGCCAAGGACATAATCGGAGGTCTCGAGTCCTGGGCCCAAGATGTGGATCAAAACTTCCCGACCTACTGA |
| Protein: MDSNGGGEVCRILRELKTLKASKSDIERRISDLEAQLRELNLNNGTVVPNGSSCTLMSTVDSGFGHGLSADMIYRYSRQLLIPSFGVQAQSNLLKSSILVVGAGGLGSPALLYLAACGVGRLGIVDHDVVELNNMHRQIIHTEAYIGQPKVKSAAASCRSINSTIQIVEHKEALCASNALEILSKYDIIVDATDNAPSRYMISDCCVVLGKPLVSGAAVGLEGQLTVYNYNGGPCYRCLFPTPPPTTACQRCSDSGVLGVVPGVIGVLQALETIKIAAAVGEPLSGRMLLFDALSARIRIVKIRGRSLQCEVCGENTAFTQKQFSEFDYENFTQSPLSTSPLKLNLLPADSRISSKEFKERIVNGEANVLVDVRPPHHYKIVSLPGSVNIPLSRLESRLPEISSFLNETKERKALNPDSVPSLYVICRRGNDSQRAVQLLHKMGFTIAKDIIGGLESWAQDVDQNFPTY |